Garden bean cultivar H25101

ABSTRACT

A novel garden bean cultivar, designated H25101, is disclosed. The invention relates to the seeds of garden bean cultivar H25101, to the plants of garden bean line H25101 and to methods for producing a bean plant by crossing the cultivar H25101 with itself or another bean line. The invention further relates to methods for producing a bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar H25101.

BACKGROUND OF THE INVENTION

The present invention relates to a new and distinctive garden beancultivar (Phaseolus vulgaris), designated H25101. All publications citedin this application are herein incorporated by reference.

There are numerous steps in the development of any novel, desirableplant germplasm. Plant breeding begins with the analysis and definitionof problems and weaknesses of the current germplasm, the establishmentof program goals, and the definition of specific breeding objectives.The next step is selection of germplasm that possesses the traits tomeet the program goals. The goal is to combine in a single variety animproved combination of desirable traits from the parental germplasm.

In beans, these important traits may include fresh pod yield, higherseed yield, resistance to diseases and insects, better stems and roots,tolerance to drought and heat, and better agronomic quality. Withmechanical harvesting of many crops, uniformity of plantscharacteristics such as germination and stand establishment, growthrate, maturity and plant height is important.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, recurrent selection andbackcross breeding.

The complexity of inheritance influences choice of the breeding method.Backcross breeding is used to transfer one or a few favorable genes fora highly heritable trait into a desirable cultivar. This approach hasbeen used extensively for breeding disease-resistant cultivars.Nevertheless, it is also suitable for the adjustment and selection ofmorphological character, color characteristics and simply inheritedquantitative characters. Various recurrent selection techniques are usedto improve quantitatively inherited traits controlled by numerous genes.The use of recurrent selection in self-pollinating crops depends on theease of pollination, the frequency of successful hybrids from eachpollination and the number of hybrid offspring from each successfulcross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested and compared toappropriate standards in environments representative of the commercialtarget area(s) for three or more years. The best lines are candidatesfor new commercial cultivars; those still deficient in a few traits maybe used as parents to produce new populations for further selection.

These processes, which lead to the final step of marketing anddistribution, usually take from eight to twelve years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior plant is to observe its performancerelative to other experimental plants and to a widely grown standardcultivar. If a single observation is inconclusive, replicatedobservations provide a better estimate of its genetic worth.

The goal of garden bean plant breeding is to develop new, unique andsuperior garden bean cultivars. The breeder initially selects andcrosses two or more parental lines, followed by repeated selfing andselection, producing many new genetic combinations. The breeder cantheoretically generate billions of different genetic combinations viacrossing, selfing and mutations.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions and further selections arethen made during and at the end of the growing season. The cultivarsthat are developed are unpredictable. This unpredictability is becausethe breeder's selection occurs in unique environments with no control atthe DNA level (using conventional breeding procedures), and withmillions of different possible genetic combinations being generated. Abreeder of ordinary skill in the art cannot predict the final resultinglines he develops, except possibly in a very gross and general fashion.This unpredictability results in the expenditure of large amounts ofresearch monies to develop superior new garden bean cultivars.

The development of new garden bean cultivars requires the developmentand selection of garden bean varieties, the crossing of these varietiesand the evaluation of the crosses.

Pedigree breeding and recurrent selection breeding methods are used todevelop cultivars from breeding populations. Breeding programs combinedesirable traits from two or more cultivars or various broad-basedsources into breeding pools from which cultivars are developed byselfing and selection of desired phenotypes.

Pedigree breeding is used commonly for the improvement ofself-pollinating crops or inbred lines of cross-pollinating crops. Twoparents that possess favorable, complementary traits are crossed toproduce an F₁. An F₂ population is produced by selfing one or severalF₁s or by intercrossing two F₁s (sib mating). Selection of the bestindividuals may begin in the F₂ population; then, beginning in the F₃,the best individuals in the best families are selected. Replicatedtesting of families, or hybrid combinations involving individuals ofthese families, often follows in the F₄ generation to improve theeffectiveness of selection for traits with low heritability. At anadvanced stage of inbreeding (i.e., F₆ and F₇), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new cultivars.

Mass and recurrent selections can be used to improve populations ofeither self- or cross-pollinating crops. A genetically variablepopulation of heterozygous individuals is either identified, or created,by intercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued.

Backcross breeding has been used to transfer genes for a simplyinherited, highly heritable trait into a desirable homozygous cultivaror inbred line which is the recurrent parent. The source of the trait tobe transferred is called the donor parent. The resulting plant isexpected to have the attributes of the recurrent parent (e.g., cultivar)and the desirable trait transferred from the donor parent. After theinitial cross, individuals possessing the phenotype of the donor parentare selected and repeatedly crossed (backcrossed) to the recurrentparent.

The single-seed descent procedure in the strict sense refers to plantinga segregating population, harvesting a sample of one seed per plant, andusing the one-seed sample to plant the next generation. When thepopulation has been advanced from the F₂ to the desired level ofinbreeding, the plants from which lines are derived will each trace todifferent F₂ individuals. The number of plants in a population declineseach generation due to failure of some seeds to germinate or some plantsto produce at least one seed. As a result, not all of the F₂ plantsoriginally sampled in the population will be represented by a progenywhen generation advance is completed.

In a multiple-seed procedure, garden bean breeders commonly harvest oneor more pods from each plant in a population and thresh them together toform a bulk. Part of the bulk is used to plant the next generation andpart is put in reserve. The procedure has been referred to as modifiedsingle-seed descent or the pod-bulk technique.

The multiple-seed procedure has been used to save labor at harvest. Itis considerably faster to thresh pods with a machine than to remove oneseed from each by hand for the single-seed procedure. The multiple-seedprocedure also makes it possible to plant the same number of seeds of apopulation each generation of inbreeding. Enough seeds are harvested tomake up for those plants that did not germinate or produce seed.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Principles of Plant Breeding, John Wiley and Son, pp.115-161, 1960; Allard, 1960; Fehr, 1987).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, the grower, processor and consumer for specialadvertising and marketing, altered seed and commercial productionpractices, and new product utilization. The testing preceding release ofa new cultivar should take into consideration research and developmentcosts as well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Garden bean, Phaseolus vulgaris L., is an important and valuablevegetable crop. Thus, a continuing goal of garden bean plant breeders isto develop stable, high yielding garden bean cultivars that areagronomically sound. The reasons for this goal are obviously to maximizethe amount of yield produced on the land. To accomplish this goal, thegarden bean breeder must select and develop garden bean plants that havetraits that result in superior cultivars.

The foregoing examples of the related art and limitations relatedtherewith are intended to be illustrative and not exclusive. Otherlimitations of the related art will become apparent to those of skill inthe art upon a reading of the specification.

SUMMARY OF THE INVENTION

The following embodiments and aspects thereof are described inconjunction with systems, tools and methods which are meant to beexemplary, not limiting in scope. In various embodiments, one or more ofthe above-described problems have been reduced or eliminated, whileother embodiments are directed to other improvements.

According to the invention, there is provided a novel garden beancultivar, designated H25101. This invention thus relates to the seeds ofgarden bean cultivar H25101, to the plants or part(s) thereof of gardenbean cultivar H25101, to plants or part(s) thereof having all thephenotypic and morphological characteristics of garden bean cultivarH25101 and to plant or part(s) thereof having the phenotypic andmorphological characteristics of garden bean cultivar H25101 listed inTable 1 as determined at the 5% significance level when grown in thesame environmental conditions. Plant parts of the garden bean cultivarof the present invention are also provided such as, i.e., pollenobtained from the plant cultivar and an ovule obtained from the plantcultivar.

In another aspect, the present invention provides regenerable cells foruse in tissue culture of bean cultivar H25101. The tissue culture willpreferably be capable of regenerating plants having the physiologicaland morphological characteristics of bean cultivar H25101. Preferably,the cells of such tissue culture will be embryos, meristematic cells,seeds, callus, pollen, leaves, anthers, pistils, roots, root tips, pods,flowers and stems. Protoplasts produced from such tissue culture arealso included in the present invention. The bean plants regenerated fromthe tissue culture are also part of the invention.

Also included in the invention are methods for producing a bean plantproduced by crossing bean cultivar H25101 with itself or another beancultivar. When crossed with itself, i.e. when crossed with another beancultivar H25101 plant or self-pollinated, bean cultivar H25101 will beconserved. When crossed with another, different bean plant, an F₁ hybridseed is produced. F₁ hybrid seeds and plants produced by growing saidhybrid seeds are included in the present invention. A method forproducing an F₁ hybrid bean seed comprising crossing a bean cultivarH25101 plant with a different bean plant and harvesting the resultanthybrid bean seed are also part of the invention. The hybrid bean seedproduced by the method comprising crossing a bean cultivar H25101 plantwith a different bean plant and harvesting the resultant hybrid beanseed, are included in the invention, as are the hybrid bean plant orpart(s) thereof, and seeds produced by growing said hybrid bean seed.

In another aspect, the present invention provides transformed H25101bean cultivar plants or part(s) thereof that have been transformed sothat its genetic material contains one or more transgenes, preferablyoperably linked to one or more regulatory elements. Also, the inventionprovides methods for producing a bean plant containing in its geneticmaterial one or more transgenes, preferably operably linked to one ormore regulatory elements, by crossing transformed H25101 bean cultivarplants with either a second plant of another bean cultivar, or a nontransformed H25101 bean cultivar, so that the genetic material of theprogeny that results from the cross contains the transgene(s),preferably operably linked to one or more regulatory elements. Theinvention also provides methods for producing a bean plant that containsin its genetic material one or more transgene(s), wherein the methodcomprises crossing the cultivar H25101 with a second bean cultivar ofanother bean cultivar which contains one or more transgene(s) operablylinked to one or more regulatory element(s) so that the genetic materialof the progeny that results from the cross contains the transgene(s)operably linked to one or more regulatory element(s). Transgenic beancultivars, or part(s) thereof produced by the methods are in the scopeof the present invention.

More specifically, the invention comprises methods for producing a malesterile bean plant, an herbicide resistant bean plant, an insectresistant bean plant, a disease resistant bean plant, a water stresstolerant bean plant, a heat stress tolerant bean plant, and a bean plantwith improved shelf-life. Said methods comprise transforming a beancultivar H25101 plant with a nucleic acid molecule that confers malesterility, herbicide resistance, insect resistance, disease resistance,water stress tolerance, heat stress tolerance, or improved shelf life,respectively. The transformed bean plants, or part(s) thereof, obtainedfrom the provided methods, including a male sterile bean plant, anherbicide resistant bean plant, an insect resistant bean plant, adisease resistant bean plant, a bean plant tolerant to water stress, abean plant tolerant to heat stress or a bean plant with improvedshelf-life are included in the present invention. For the presentinvention and the skilled artisan, disease is understood to be fungaldiseases, viral diseases, bacterial diseases or other plant pathogenicdiseases and a disease resistant plant will encompass a plant resistantto fungal, viral, bacterial and other plant pathogens.

In another aspect, the present invention provides for methods ofintroducing one or more desired trait(s) into bean cultivar H25101 andplants obtained from such methods. The desired trait(s) may be, but notexclusively, a single gene, preferably a dominant but also a recessiveallele. Preferably, the transferred gene or genes will confer suchtraits as male sterility, herbicide resistance, insect resistance,resistance to bacterial, fungal, or viral disease, increased leafnumber, improved shelf-life, and tolerance to water stress or heatstress. The gene or genes may be naturally occurring gene(s) ortransgene(s) introduced through genetic engineering techniques. Themethod for introducing the desired trait(s) is preferably a backcrossingprocess making use of a series of backcrosses to bean cultivar H25101during which the desired trait(s) is maintained by selection.

When using a transgene, the trait is generally not incorporated intoeach newly developed line/cultivar such as bean cultivar H25101 bydirect transformation. Rather, the more typical method used by breedersof ordinary skill in the art to incorporate the transgene is to take aline already carrying the transgene and to use such line as a donor lineto transfer the transgene into the newly developed line. The same wouldapply for a naturally occurring trait. The backcross breeding processcomprises the following steps: (a) crossing bean cultivar H25101 plantswith plants of another cultivar that comprise the desired trait(s); (b)selecting the F₁ progeny plants that have the desired trait(s); (c)crossing the selected F₁ progeny plants with bean cultivar H25101 plantsto produce backcross progeny plants; (d) selecting for backcross progenyplants that have the desired trait(s) and physiological andmorphological characteristics of bean cultivar H25101 to produceselected backcross progeny plants; and (e) repeating steps (c) and (d)one, two, three, four, five six, seven, eight, nine or more times insuccession to produce selected, second, third, fourth, fifth, sixth,seventh, eighth, ninth or higher backcross progeny plants that comprisethe desired trait(s) and the physiological and morphologicalcharacteristics of bean cultivar H25101 as determined in Table one at a5% significance level when grown in the same environmental conditions.The bean plants produced by the methods are also part of the invention.Backcrossing breeding methods, well-known for a man skilled in the artof plant breeding, will be further developed in subsequent parts of thespecification.

In a preferred embodiment, the present invention provides methods forincreasing and producing bean cultivar H25101 seed, whether by crossinga first parent bean cultivar plant with a second parent bean cultivarplant and harvesting the resultant bean seed, wherein both said firstand second parent bean cultivar plant are the bean cultivar H25101 or byplanting a bean seed of the bean cultivar H25101, growing a beancultivar H25101 plant from said seed, controlling a self pollination ofthe plant where the pollen produced by a grown bean cultivar H25101plant pollinates the ovules produced by the very same bean cultivarH25101 grown plant and harvesting the resultant seed.

The invention further provides methods for developing bean cultivars ina bean breeding program using plant breeding technique includingrecurrent selection, backcrossing, pedigree breeding, molecular markers(Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms(RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily PrimedPolymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting(DAF), Sequence Characterized Amplified Regions (SCARs). AmplifiedFragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats(SSRs) which are also referred to as Microsatellites, etc.) enhancedselection, genetic marker enhanced selection and transformation. Seeds,bean plants, and part(s) thereof produced by such breeding methods arealso part of the invention.

In addition to the exemplary aspects and embodiments described above,further aspects and embodiments will become apparent by study of thefollowing descriptions.

DEFINITIONS

In the description and tables that follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

Allele. An allele is any of one or more alternative forms of a genewhich relate to one trait or characteristic. In a diploid cell ororganism, the two alleles of a given gene occupy corresponding loci on apair of homologous chromosomes.

Backcrossing. Backcrossing is a process in which a breeder repeatedlycrosses hybrid progeny back to one of the parents, for example, a firstgeneration hybrid F₁ with one of the parental genotypes of the F₁hybrid.

Bean Yield (Tons/Acre). The yield in tons/acre is the actual yield ofthe bean pods at harvest.

Determinate plant. A determinate plant will grow to a fixed number ofnodes while an indeterminate plant continues to grow during the season.

Emergence. The rate that the seed germinates and sprouts out of theground.

Essentially all the physiological and morphological characteristics. Aplant having essentially all the physiological and morphologicalcharacteristics means a plant having the physiological and morphologicalcharacteristics, except for the characteristics derived from theconverted gene.

Field holding ability. A bean plant that has field holding ability meansa plant having pods those remain smooth and retain their color evenafter the seed is almost fully developed.

Immunity to disease(s) and or insect(s). A bean plant which is notsubject to attack or infection by specific disease(s) and or insect(s)is considered immune.

Intermediate Resistance to disease(s) and or insect(s): A bean plantthat restricts the growth and development of specific disease(s) and orinsect(s), but may exhibit a greater range of symptoms or damagecompared to resistant plants. Intermediate resistant plants will usuallyshow less severe symptoms or damage than susceptible plant varietieswhen grown under similar environmental conditions and/or specificdisease(s) and or insect(s) pressure, but may have heavy damage underheavy pressure. Intermediate resistant bean plants are not immune to thedisease(s) and or insect(s).

Machine harvestable bush. A machine harvestable bush means a bean plantthat stands with pods off the ground. The pods can be removed by amachine from the plant without leaves and other plant parts.

Maturity. A maturity under 53 days is considered early while maturitybetween 54-59 days is considered average or medium and maturity of 60 ormore days would be late.

Maturity date. Plants are considered mature when the pods have reachedtheir maximum allowable seed size and sieve size for the specific useintended. This can vary for each end user, e.g., processing at differentstages of maturity would be required for different types of consumerbeans such as “whole pack,” “cut” or “french style”. The number of daysare calculated from a relative planting date which depends on daylength, heat units and environmental other factors.

Plant adaptability. A plant having good plant adaptability means a plantthat will perform well in different growing conditions and seasons.

Plant architecture. Plant architecture is the shape of the overall plantwhich can be tall-narrow, short-wide, medium height, medium width.

Plant cell. As used herein, the term “plant cell” includes plant cellswhether isolated, in tissue culture or incorporated in a plant or plantpart.

Plant habit. A plant can be erect (upright) to sprawling on the ground.

Plant height. Plant height is taken from the top of the soil to the topnode of the plant and is measured in inches.

Plant part. As used herein, the term “plant part” includes leaves,stems, roots, seed, embryos, pollen, ovules, flowers, root tips,anthers, tissue, cells, pods and the like.

Pod set height. The pod set height is the location of the pods withinthe plant. The pods can be high (near the top), low (near the bottom) ormedium (in the middle) of the plant.

Regeneration. Regeneration refers to the development of a plant fromtissue culture.

Resistance to disease(s) and or insect(s). A bean plant that restrictsthe growth and development of specific disease(s) and or insect(s) undernormal disease(s) and or insect(s) attack pressure when compared tosusceptible plants. These bean plants can exhibit some symptoms ordamage under heavy disease(s) and or insect(s) pressure. Resistant beanplants are not immune to the disease(s) and or insect(s).

RHS. RHS refers to the Royal Horticultural Society of England whichpublishes an official botanical color chart quantitatively identifyingcolors according to a defined numbering system. The chart may bepurchased from Royal Hort. Society Enterprise Ltd. RHS Garden; Wisley,Woking; Surrey GU236QB, UK.

Sieve Size (sv). Sieve size 1 means pods that fall through a sievegrader which culls out pod diameters of 4.76 mm through 5.76 mm. Sievesize 2 means pods that fall through a sieve grader which culls out poddiameters of 5.76 mm through 7.34 mm. Sieve size 3 means pods that fallthrough a sieve grader which culls out pod diameters of 7.34 mm through8.34 mm. Sieve size 4 means pods that fall through a sieve grader whichculls out pod diameters of 8.34 mm through 9.53 mm. Sieve size 5 meanspods that fall through a sieve grader which culls out pod diameters of9.53 mm through 10.72 mm. Sieve size 6 means pods that fall through asieve grader that will cull out pod diameters of 10.72 mm or larger.

Single gene converted (conversion). Single gene converted (conversion)plants refers to plants which are developed by a plant breedingtechnique called backcrossing wherein essentially all of the desiredmorphological and physiological characteristics of a variety arerecovered in addition to the single gene transferred into the varietyvia the backcrossing technique or via genetic engineering.

Slow seed development. Beans having slow seed development develop seedslowly even after the pods are full sized. This characteristic will giveto the cultivar its field holding ability.

Susceptible to disease(s) and or insect(s). A bean plant that issusceptible to disease(s) and or insect(s) is defined as a bean plantthat has the inability to restrict the growth and development ofspecific disease(s) and or insect(s). Plants that are susceptible willshow damage when infected and are more likely to have heavy damage undermoderate levels of specific disease(s) and or insect(s).

DETAILED DESCRIPTION OF THE INVENTION

Garden bean cultivar H25101 has superior characteristics and wasdeveloped from an initial cross that was made in a greenhouse in SunPrairie, Wis., in October of 2000. The cross was between two proprietarylines under stake numbers W5170 (female) and W5127 (male). The F₁generation was harvested April 2001 in the Sun Prairie, Wis., in plotW5576. The F₂ selection was made July 2001 near Coloma, Wis. in plotH10413. The F₃ selection was made February 2002 near Los Mochis, Mexicoin plot M20860. The F₄ selection was made July 2002 near Coloma, Wis. inplot H24593. The F₅ selection was made February 2003 near Los Mochis,Mexico in plot M30363. The F₆ selection was made July 2003 near Coloma,Wis. in plot H304814. The F₇ generation was bulked March 2004 near LosMochis, Mexico in plot M42078. The F₈ generation was bulked September2004 at San Juan Bautista, Calif. in plot C406306. The F₉ generation wasbulked March 2005 near Los Mochis, Mexico in plot M54501-518. The linewas designated H25101.

Garden bean cultivar H25101 is similar to garden bean cultivar‘Hystyle’. While similar to garden bean cultivar ‘Hystyle’, there aresignificant differences including garden bean cultivar H25101 has podsthat are darker green than the pods of garden bean cultivar ‘Hystyle’.In addition, garden bean cultivar H25101 does not have the recessive“persistent green” gene contained in garden bean cultivar ‘Hystyle’.

Garden bean cultivar H25101 is a 55 day medium maturity bean withuniform medium dark-green pods on an upright plant structure (habit).The pods are straight and smooth and are borne in the upper one-half ofthe plant. The majority of the pods are in the 4-5 sieves range. Theleaves are medium in size with a dull medium green color. Garden beancultivar H25101 is a determinate plant and is resistant to Bean CommonMosaic Virus (BCMV I-gene) and has intermediate resistance to Beet CurlyTop Virus and Psuedomonas syringae pv syringae (Bacterial brown spot).

Some of the selection criteria used for various generations include: podappearance and length, bean yield, pod set height, emergence, maturity,plant architecture, habit and height, seed yield and quality and diseaseresistance.

Bean Common Mosaic Virus resistance is a desired trait for a beanvariety. The disease occurs worldwide causing low quality of the harvestproduct and losses from 80% to 100% by reduction of yield. It is mostlytransmitted by aphids during the growing season, but can also be spreadby pollen or mechanically. The leaves develop mosaic patterns in whichirregular light and dark green patches are intermixed. Malformation andyellow dots may also be produced, often causing growth reduction. Theplant may be dwarfed and pod and seed yield reduced. Severe necrosis mayoccur and the plant may die if infected while young. Systemic necrosis,in which the roots and shoots become blackened, appears in cultivarshaving a dominant resistance gene (hypersensitive resistance mechanism).The systemic necrosis may spread to higher leaves without killing themor may be concentrated in the vascular parts of the stem, eventuallyleading to the death of all or part of the plant. When infection occurslate in plant development, parts of the plant may die and many pods mayshow brown discoloration in the pod wall and pod suture as a result ofvascular necrosis.

Garden bean cultivar H25101 has shown uniformity and stability for thetraits, as described in the following Variety Description Information.It has been self-pollinated a sufficient number of generations withcareful attention to uniformity of plant type. The cultivar has beenincreased with continued observation for uniformity. No variant traitshave been observed or are expected in garden bean cultivar H25101.

Garden bean cultivar H25101 has the following morphologic and othercharacteristics (based primarily on data collected at Immokalee, Fla.,Crossville, Tenn., and Sun Prairie, Wis.).

TABLE 1 VARIETY DESCRIPTION INFORMATION Market Maturity: Days to ediblepods: 55 Number of days earlier than ‘Hystyle’:  0 Plant: Habit:Determinate Height: 49 cm Taller than ‘Hystyle’ by 2 cm Spread: 58 cmNarrower than ‘Hystyle’ by 0 cm Pod position: Medium-high Bush form:High Bush Leaves: Surface: Dull Size: Medium Color: Medium-greenAnthocyanin Pigment: Flowers: Absent Stems: Absent Pods: Absent Seeds:Absent Leaves: Absent Petioles: Absent Peduncles: Absent Nodes: AbsentFlower Color: Color of standard: White Color of wings: White Color ofkeel: White Pods (edible maturity): Exterior color: Medium dark-greenDry pod color: Buckskin Cross section pod shape: Round-creasebackCreaseback: Present Pubescence: Sparse Constriction: None Spur length:12 mm Fiber: Sparse Number of seeds/pods:  6 Suture string: Absent Seeddevelopment: Very slow Machine harvest: Adapted Distribution of sievesize at optimum maturity: 10% 7.34 mm to 8.34 mm - Sieve 3 50% 8.34 mmto 9.53 mm - Sieve 4 40% 9.53 mm to 10.72 mm - Sieve 5 Seed Color: Seedcoat luster: Shiny Seed coat: Monochrome Primary color: White Seed coatpattern: Solid Hilar ring: Absent Seed Shape and Size: Hilum View:Elliptical Cross Section: Oval Side view: Oval to oblong Seed Size(g/100 seeds): 28 1 gm/100 seeds lighter than ‘Hystyle’ DiseaseResistance: Bean Common Mosaic Virus (BCMV I gene): Resistant Beet CurlyTop Virus: Intermediate Resistant Psuedomonas syringae pv syringaeIntermediate (Bacterial Brown Spot): Resistant

Further Embodiments of the Invention

This invention also is directed to methods for producing a garden plantby crossing a first parent bean plant with a second parent bean plantwherein either the first or second parent bean plant is a bean plant ofthe line H25101. Further, both first and second parent bean plants cancome from cultivar H25101. When self pollinated, or crossed with anotherbean cultivar H25101 plant, the bean cultivar H25101 will be stable,while when crossed with another, different bean cultivar plant, an F₁hybrid seed is produced.

Still further, this invention also is directed to methods for producingan H25101-derived bean plant by crossing cultivar H25101 with a secondbean plant and growing the progeny seed, and repeating the crossing andgrowing steps with the cultivar H25101-derived plant from 0 to 7 times.Thus, any such methods using the cultivar H25101 are part of thisinvention: selfing, backcrosses, hybrid production, crosses topopulations, and the like. All plants produced using cultivar H25101 asa parent are within the scope of this invention, including plantsderived from cultivar H25101. Advantageously, the cultivar is used incrosses with other, different, cultivars to produce first generation(F₁) bean seeds and plants with superior characteristics.

As used herein, the term “plant” includes plant cells, plantprotoplasts, plant cell tissue cultures from which garden bean plantscan be regenerated, plant calli, plant clumps and plant cells that areintact in plants or parts of plants, such as embryos, pollen, ovules,flowers, seeds, pods, stems, roots, anthers, pistils, root tips, leavesand the like.

As is well known in the art, tissue culture of garden bean can be usedfor the in vitro regeneration of a garden bean plant. Tissue culture ofvarious tissues of garden beans and regeneration of plants therefrom iswell known and widely published. For example, reference may be had toMcClean, P.; Grafton, K. F. (1989): “Regeneration of dry bean (Phaseolusvulgaris) via organogenesis.” Plant Sci. 60, 117-122. Mergeai, G.;Baudoin, J. P. (1990): “Development of an in vitro culture method forheart-shaped embryo in Phaseolus vulgaris.” B.I.C. Invit. Papers 33,115-116. Vanderwesthuizen, A. J.; Groenewald, E. G. (1990): “RootFormation and Attempts to Establish Morphogenesis in Callus Tissues ofBeans (Phaseolus-vulgaris L.).” S. Afr. J. Bot 56 (2, April), 271-273.Benedicic, D., et al. (1990): “The regeneration of Phaseolus vulgaris L.plants from meristem culture.” Abst. 5th I.A.P.T.C. Cong. 1, 91(#A3-33). Genga, A.; Allavena, A. (1990): “Factors affectingmorphogenesis from immature cotyledons of Phaseolus coccineus L.” Abst.5th I.A.P.T.C. Cong. 1, 101 (#A3-75). Vaquero, F., et al. (1990): “Plantregeneration and preliminary studies on transformation of Phaseoluscoccineus.” Abst. 5th I.A.P.T.C. Cong. 1, 106 (#A3-93). Franklin, C. I.,et al. (1991): “Plant Regeneration from Seedling Explants of Green Bean(Phaseolus-Vulgaris L.) via Organogenesis.” Plant Cell Tissue Org. Cult.24 (3, March), 199-206. Malik, K. A.; Saxena, P. K. (1991):“Regeneration in Phaseolus-vulgaris L.—Promotive Role ofN6-Benzylaminopurine in Cultures from Juvenile Leaves.” Planta 184(1),148-150. Genga, A.; Allavena, A. (1991): “Factors affectingmorphogenesis from immature cotyledones of Phaseolus coccineus L.” PlantCell Tissue Org. Cult 27, 189-196. Malik, K. A.; Saxena, P. K. (1992):“Regeneration in Phaseolus vulgaris L.—High-Frequency Induction ofDirect Shoot Formation in Intact Seedlings by N-6-Benzylaminopurine andThidiazuron.” 186 (3, February), 384-389. Malik, K. A.; Saxena, P. K.(1992): “Somatic Embryogenesis and Shoot Regeneration from IntactSeedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P.coccineus L., and P. wrightii L.” Pl. Cell. Rep. 11(3, April), 163-168.Chavez, J., et al. (1992): “Development of an in vitro culture methodfor heart shaped embryo in Phaseolus polyanthus.” B.I.C. Invit. Papers35, 215-216. Munoz-Florez, L. C., et al. (1992): “Finding out anefficient technique for inducing callus from Phaseolus microspores.”B.I.C. Invit. Papers 35, 217-218. Vaquero, F., et al. (1993): “A Methodfor Long-Term Micropropagation of Phaseolus coccineus L.”L. Pl. Cell.Rep. 12 (7-8, May), 395-398. Lewis, M. E.; Bliss, F. A. (1994): “TumorFormation and beta-Glucuronidase Expression in Phaseolus vulgaris L.Inoculated with Agrobacterium Tumefaciens.” Journal of the AmericanSociety for Horticultural Science 119 (2, March), 361-366. Song, J. Y.,et al. (1995): “Effect of auxin on expression of the isopentenyltransferase gene (ipt) in transformed bean (Phaseolus vulgaris L.)single-cell clones induced by Agrobacterium tumefaciens C58.” J. PlantPhysiol. 146 (1-2, May), 148-154. It is clear from the literature thatthe state of the art is such that these methods of obtaining plants areroutinely used and have a very high rate of success. Thus, anotheraspect of this invention is to provide cells which upon growth anddifferentiation produce bean plants having the physiological andmorphological characteristics of garden bean cultivar H25101.

As used herein, the term “tissue culture” indicates a compositioncomprising isolated cells of the same or a different type or acollection of such cells organized into parts of a plant. Exemplarytypes of tissue cultures are protoplasts, calli, plant clumps, and plantcells that can generate tissue culture that are intact in plants orparts of plants, such as embryos, pollen, flowers, seeds, pods, leaves,stems, roots, root tips, anthers, pistils and the like. Means forpreparing and maintaining plant tissue culture are well known in theart. By way of example, a tissue culture comprising organs has been usedto produce regenerated plants. U.S. Pat. Nos. 5,959,185; 5,973,234 and5,977,445 describe certain techniques, the disclosures of which areincorporated herein by reference.

With the advent of molecular biological techniques that have allowed theisolation and characterization of genes that encode specific proteinproducts, scientists in the field of plant biology developed a stronginterest in engineering the genome of plants to contain and expressforeign genes, or additional, or modified versions of native, orendogenous, genes (perhaps driven by different promoters) in order toalter the traits of a plant in a specific manner. Such foreignadditional and/or modified genes are referred to herein collectively as“transgenes”. Over the last fifteen to twenty years several methods forproducing transgenic plants have been developed and the presentinvention, in particular embodiments, also relates to transformedversions of the claimed variety or line.

Plant transformation involves the construction of an expression vectorwhich will function in plant cells. Such a vector comprises DNAcomprising a gene under control of, or operatively linked to, aregulatory element (for example, a promoter). The expression vector maycontain one or more such operably linked gene/regulatory elementcombinations. The vector(s) may be in the form of a plasmid and can beused alone or in combination with other plasmids to provide transformedgarden bean plants using transformation methods as described below toincorporate transgenes into the genetic material of the garden beanplant(s).

Expression Vectors for Garden Bean Transformation: Marker Genes

Expression vectors include at least one genetic marker operably linkedto a regulatory element (a promoter, for example) that allowstransformed cells containing the marker to be either recovered bynegative selection, i.e., inhibiting growth of cells that do not containthe selectable marker gene, or by positive selection, i.e., screeningfor the product encoded by the genetic marker. Many commonly usedselectable marker genes for plant transformation are well known in thetransformation arts, and include, for example, genes that code forenzymes that metabolically detoxify a selective chemical agent which maybe an antibiotic or an herbicide, or genes that encode an altered targetwhich is insensitive to the inhibitor. A few positive selection methodsare also known in the art.

One commonly used selectable marker gene for plant transformation is theneomycin phosphotransferase II (nptII) gene which, when under thecontrol of plant regulatory signals, confers resistance to kanamycin.Fraley et al., Proc. Natl. Acad. Sci. USA, 80:4803 (1983). Anothercommonly used selectable marker gene is the hygromycinphosphotransferase gene which confers resistance to the antibiotichygromycin. Vanden Eizen et al., Plant Mol. Biol., 5:299 (1985).

Additional selectable marker genes of bacterial origin that conferresistance to antibiotics include gentamycin acetyl transferase,streptomycin phosphotransferase and aminoglycoside-3′-adenyltransferase, the bleomycin resistance determinant (Hayford et al., PlantPhysiol. 86:1216 (1988), Jones et al., Mol. Gen. Genet., 210:86 (1987),Svab et al., Plant Mol. Biol. 14:197 (1990), Hille et al., Plant Mol.Biol. 7:171 (1986)). Other selectable marker genes confer resistance toherbicides such as glyphosate, glufosinate or bromoxynil (Comai et al.,Nature 317:741-744 (1985), Gordon-Kamm et al., Plant Cell 2:603-618(1990) and Stalker et al., Science 242:419-423 (1988)).

Other selectable marker genes for plant transformation are not ofbacterial origin. These genes include, for example, mouse dihydrofolatereductase, plant 5-enolpyruvylshikimate-3-phosphate synthase and plantacetolactate synthase. Eichholtz et al., Somatic Cell Mol. Genet 13:67(1987), Shah et al., Science 233:478 (1986), Charest et al., Plant CellRep. 8:643 (1990).

Another class of marker genes for plant transformation requiresscreening of presumptively transformed plant cells rather than directgenetic selection of transformed cells for resistance to a toxicsubstance such as an antibiotic. These genes are particularly useful toquantify or visualize the spatial pattern of expression of a gene inspecific tissues and are frequently referred to as reporter genesbecause they can be fused to a gene or gene regulatory sequence for theinvestigation of gene expression. Commonly used genes for screeningpresumptively transformed cells include β-glucuronidase (GUS),β-galactosidase, luciferase and chloramphenicol acetyltransferase(Jefferson, R. A., Plant Mol. Biol. Rep. 5:387 (1987), Teeri et al.,EMBO J. 8:343 (1989), Koncz et al., Proc. Natl. Acad. Sci. USA 84:131(1987), DeBlock et al., EMBO J. 3:1681 (1984)).

In vivo methods for visualizing GUS activity that do not requiredestruction of plant tissue are available. However, these in vivomethods for visualizing GUS activity have not proven useful for recoveryof transformed cells because of low sensitivity, high fluorescentbackgrounds and limitations associated with the use of luciferase genesas selectable markers.

A gene encoding Green Fluorescent Protein (GFP) has been utilized as amarker for gene expression in prokaryotic and eukaryotic cells (Chalfieet al., Science 263:802 (1994)). GFP and mutants of GFP may be used asscreenable markers.

Expression Vectors for Garden Bean Transformation: Promoters

Genes included in expression vectors must be driven by a nucleotidesequence comprising a regulatory element, for example, a promoter.Several types of promoters are well known in the transformation arts asare other regulatory elements that can be used alone or in combinationwith promoters.

As used herein, “promoter” includes reference to a region of DNAupstream from the start of transcription and involved in recognition andbinding of RNA polymerase and other proteins to initiate transcription.A “plant promoter” is a promoter capable of initiating transcription inplant cells. Examples of promoters under developmental control includepromoters that preferentially initiate transcription in certain tissues,such as leaves, roots, seeds, fibers, xylem vessels, tracheids, orsclerenchyma. Such promoters are referred to as “tissue-preferred”.Promoters that initiate transcription only in a certain tissue arereferred to as “tissue-specific”. A “cell-type” specific promoterprimarily drives expression in certain cell types in one or more organs,for example, vascular cells in roots or leaves. An “inducible” promoteris a promoter which is under environmental control. Examples ofenvironmental conditions that may effect transcription by induciblepromoters include anaerobic conditions or the presence of light.Tissue-specific, tissue-preferred, cell type specific, and induciblepromoters constitute the class of “non-constitutive” promoters. A“constitutive” promoter is a promoter that is active under mostenvironmental conditions.

A. Inducible Promoters—An inducible promoter is operably linked to agene for expression in garden bean. Optionally, the inducible promoteris operably linked to a nucleotide sequence encoding a signal sequencewhich is operably linked to a gene for expression in garden bean. Withan inducible promoter the rate of transcription increases in response toan inducing agent.

Any inducible promoter can be used in the instant invention. See Ward etal., Plant Mol. Biol. 22:361-366 (1993). Exemplary inducible promotersinclude, but are not limited to, that from the ACEI system whichresponds to copper (Mett et al., Proc. Natl. Acad. Sci. USA 90:4567-4571(1993)); In2 gene from maize which responds to benzenesulfonamideherbicide safeners (Gatz et al., Mol. Gen. Genetics 243:32-38 (1994)) orTet repressor from Tn10 (Gatz et al., Mol. Gen. Genetics 227:229-237(1991)). A particularly preferred inducible promoter is a promoter thatresponds to an inducing agent to which plants do not normally respond.An exemplary inducible promoter is the inducible promoter from a steroidhormone gene, the transcriptional activity of which is induced by aglucocorticosteroid hormone (Schena et al., Proc. Natl. Acad. Sci. USA88:0421 (1991)).

B. Constitutive Promoters—A constitutive promoter is operably linked toa gene for expression in garden bean or the constitutive promoter isoperably linked to a nucleotide sequence encoding a signal sequencewhich is operably linked to a gene for expression in garden bean.

Many different constitutive promoters can be utilized in the instantinvention. Exemplary constitutive promoters include, but are not limitedto, the promoters from plant viruses such as the 35S promoter from CaMV(Odell et al., Nature 313:810-812 (1985)) and the promoters from suchgenes as rice actin (McElroy et al., Plant Cell 2: 163-171 (1990));ubiquitin (Christensen et al., Plant Mol. Biol. 12:619-632 (1989) andChristensen et al., Plant Mol. Biol. 18:675-689 (1992)); pEMU (Last etal., Theor. Appl. Genet. 81:581-588 (1991)); MAS (Velten et al., EMBO J.3:2723-2730 (1984)) and maize H3 histone (Lepetit et al., Mol. Gen.Genetics 231:276-285 (1992) and Atanassova et al., Plant Journal 2 (3):291-300 (1992)). The ALS promoter, Xba1/Ncol fragment 5′ to the Brassicanapus ALS3 structural gene (or a nucleotide sequence similarity to saidXba1/Ncol fragment), represents a particularly useful constitutivepromoter. See PCT application WO 96/30530.

C. Tissue-specific or Tissue-preferred Promoters—A tissue-specificpromoter is operably linked to a gene for expression in garden bean.Optionally, the tissue-specific promoter is operably linked to anucleotide sequence encoding a signal sequence which is operably linkedto a gene for expression in garden bean. Plants transformed with a geneof interest operably linked to a tissue-specific promoter produce theprotein product of the transgene exclusively, or preferentially, in aspecific tissue.

Any tissue-specific or tissue-preferred promoter can be utilized in theinstant invention. Exemplary tissue-specific or tissue-preferredpromoters include, but are not limited to, a root-preferred promotersuch as that from the phaseolin gene (Murai et al., Science 23:476-482(1983) and Sengupta-Gopalan et al., Proc. Natl. Acad. Sci. USA82:3320-3324 (1985)); a leaf-specific and light-induced promoter such asthat from cab or rubisco (Simpson et al., EMBO J. 4(11):2723-2729 (1985)and Timko et al., Nature 318:579-582 (1985)); an anther-specificpromoter such as that from LAT52 (Twell et al., Mol. Gen. Genetics217:240-245 (1989)); a pollen-specific promoter such as that from Zm13or a microspore-preferred promoter such as that from apg (Twell et al.,Sex. Plant Reprod. 6:217-224 (1993)).

Signal Sequences for Targeting Proteins to Subcellular Compartments

Transport of protein produced by transgenes to a subcellular compartmentsuch as the chloroplast, vacuole, peroxisome, glyoxysome, cell wall ormitochondrion or for secretion into the apoplast, is accomplished bymeans of operably linking the nucleotide sequence encoding a signalsequence to the 5′ and/or 3′ region of a gene encoding the protein ofinterest. Targeting sequences at the 5′ and/or 3′ end of the structuralgene may determine during protein synthesis and processing where theencoded protein is ultimately compartmentalized.

The presence of a signal sequence directs a polypeptide to either anintracellular organelle or subcellular compartment or for secretion tothe apoplast. Many signal sequences are known in the art. See, forexample, Becker et al., Plant Mol. Biol. 20:49 (1992); Knox, C., et al.,“Structure and Organization of Two Divergent Alpha-Amylase Genes fromBarley”, Plant Mol. Biol. 9:3-17 (1987); Lerner et al., Plant Physiol.91:124-129 (1989); Fontes et al., Plant Cell 3:483-496 (1991); Matsuokaet al., Proc. Natl. Acad. Sci. 88:834 (1991); Gould et al., J. Cell.Biol. 108:1657 (1989); Creissen et al., Plant J. 2:129 (1991); Kalderon,et al., Cell 39:499-509 (1984); Steifel, et al., “Expression of a maizecell wall hydroxyproline-rich glycoprotein gene in early leaf and rootvascular differentiation”, Plant Cell 2:785-793 (1990).

Foreign Protein Genes and Agronomic Genes

With transgenic plants according to the present invention, a foreignprotein can be produced in commercial quantities. Thus, techniques forthe selection and propagation of transformed plants, which are wellunderstood in the art, yield a plurality of transgenic plants which areharvested in a conventional manner, and a foreign protein then can beextracted from a tissue of interest or from total biomass. Proteinextraction from plant biomass can be accomplished by known methods whichare discussed, for example, by Heney and Orr, Anal. Biochem. 114:92-6(1981).

According to a preferred embodiment, the transgenic plant provided forcommercial production of foreign protein is a garden bean plant. Inanother preferred embodiment, the biomass of interest is seed. For therelatively small number of transgenic plants that show higher levels ofexpression, a genetic map can be generated, primarily via conventionalRFLP, PCR and SSR analysis, which identifies the approximate chromosomallocation of the integrated DNA molecule. For exemplary methodologies inthis regard, see Glick and Thompson, Methods in Plant Molecular Biologyand Biotechnology, CRC Press, Boca Raton 269:284 (1993). Map informationconcerning chromosomal location is useful for proprietary protection ofa subject transgenic plant. If unauthorized propagation is undertakenand crosses made with other germplasm, the map of the integration regioncan be compared to similar maps for suspect plants, to determine if thelatter have a common parentage with the subject plant. Map comparisonswould involve hybridizations, RFLP, PCR, SSR and sequencing, all ofwhich are conventional techniques.

Likewise, by means of the present invention, agronomic genes can beexpressed in transformed plants. More particularly, plants can begenetically engineered to express various phenotypes of agronomicinterest. Exemplary genes implicated in this regard include, but are notlimited to, those categorized below:

1. Genes that Confer Resistance to Pests or Disease and that Encode:

A. Plant disease resistance genes. Plant defenses are often activated byspecific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with one ormore cloned resistance genes to engineer plants that are resistant tospecific pathogen strains. See, for example Jones et al., Science266:789 (1994) (cloning of the tomato Cf-9 gene for resistance toCladosporium fulvum); Martin et al., Science 262:1432 (1993) (tomato Ptogene for resistance to Pseudomonas syringae pv. tomato encodes a proteinkinase); Mindrinos et al. Cell 78:1089 (1994) (Arabidopsis RSP2 gene forresistance to Pseudomonas syringae).

B. A Bacillus thuringiensis protein, a derivative thereof or a syntheticpolypeptide modeled thereon. See, for example, Geiser et al., Gene48:109 (1986), who disclose the cloning and nucleotide sequence of a Btδ-endotoxin gene. Moreover, DNA molecules encoding δ-endotoxin genes canbe purchased from American Type Culture Collection, Manassas, Va., forexample, under ATCC Accession Nos. 40098, 67136, 31995 and 31998.

C. A lectin. See, for example, Van Damme et al., Plant Molec. Biol.24:25 (1994), who disclose the nucleotide sequences of several Cliviaminiata mannose-binding lectin genes.

D. A vitamin-binding protein such as avidin. See PCT application US93/06487 which teaches the use of avidin and avidin homologues aslarvicides against insect pests.

E. An enzyme inhibitor, for example, a protease or proteinase inhibitoror an amylase inhibitor. See, for example, Abe et al., J. Biol. Chem.262:16793 (1987) (nucleotide sequence of rice cysteine proteinaseinhibitor), Huub et al., Plant Molec. Biol. 21:985 (1993) (nucleotidesequence of cDNA encoding tobacco proteinase inhibitor I), Sumitani etal., Biosci. Biotech. Biochem. 57:1243 (1993) (nucleotide sequence ofStreptomyces nitrosporeus α-amylase inhibitor).

F. An insect-specific hormone or pheromone such as an ecdysteroid orjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock et al., Nature 344:458 (1990), of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

G. An insect-specific peptide or neuropeptide which, upon expression,disrupts the physiology of the affected pest. For example, see thedisclosure of Pratt et al., Biochem. Biophys. Res. Comm. 163:1243 (1989)(an allostatin is identified in Diploptera puntata). See also U.S. Pat.No. 5,266,317 to Tomalski et al., which discloses genes encodinginsect-specific, paralytic neurotoxins.

H. An insect-specific venom produced in nature by a snake, a wasp, etc.For example, see Pang et al., Gene 116:165 (1992), for disclosure ofheterologous expression in plants of a gene coding for a scorpioninsectotoxic peptide.

I. An enzyme responsible for a hyperaccumulation of a monoterpene, asesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivativeor another non-protein molecule with insecticidal activity.

J. An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule; forexample, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme,a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic. See PCTapplication WO 93/02197 (Scott et al.), which discloses the nucleotidesequence of a callase gene. DNA molecules which containchitinase-encoding sequences can be obtained, for example, from the ATCCunder Accession Nos. 39637 and 67152. See also Kramer et al., InsectBiochem. Molec. Biol. 23:691 (1993), who teach the nucleotide sequenceof a cDNA encoding tobacco hornworm chitinase, and Kawalleck et al.,Plant Molec. Biol. 21:673 (1993), who provide the nucleotide sequence ofthe parsley ubi4-2 polyubiquitin gene.

K. A molecule that stimulates signal transduction. For example, see thedisclosure by Botella et al., Plant Molec. Biol. 24:757 (1994), ofnucleotide sequences for mung bean calmodulin cDNA clones, and Griess etal., Plant Physiol. 104:1467 (1994), who provide the nucleotide sequenceof a maize calmodulin cDNA clone.

L. A hydrophobic moment peptide. See PCT application WO 95/16776, whichdiscloses peptide derivatives of tachyplesin which inhibit fungal plantpathogens, and PCT application WO 95/18855 which teaches syntheticantimicrobial peptides that confer disease resistance.

M. A membrane permease, a channel former or a channel blocker. Forexample, see the disclosure of Jaynes et al., Plant Sci 89:43 (1993), ofheterologous expression of a cecropin-β lytic peptide analog to rendertransgenic tobacco plants resistant to Pseudomonas solanacearum.

N. A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. See Beachy et al., Ann. Rev. Phytopathol.28:451 (1990). Coat protein-mediated resistance has been conferred upontransformed plants against alfalfa mosaic virus, cucumber mosaic virusand tobacco streak virus, potato virus X, potato virus Y, tobacco etchvirus, tobacco rattle virus and tobacco mosaic virus. Id.

O. An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect.

P. A virus-specific antibody. See, for example, Tavladoraki et al.,Nature 366:469 (1993), who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

Q. A developmental-arrestive protein produced in nature by a pathogen ora parasite. Thus, fungal endo-α-1,4-D-polygalacturonases facilitatefungal colonization and plant nutrient release by solubilizing plantcell wall homo-α-1,4-D-galacturonase. See Lamb et al., Bio/Technology10:1436 (1992).

R. A developmental-arrestive protein produced in nature by a plant. Forexample, Logemann et al., Bio/Technology 10:305 (1992), have shown thattransgenic plants expressing the barley ribosome-inactivating gene havean increased resistance to fungal disease.

S. Genes involved in the Systemic Acquired Resistance (SAR) Responseand/or the pathogenesis-related genes. Briggs, S., “Plant diseaseresistance. Grand unification system theory in sight”, Current Biology,5(2) (1995).

T. Antifungal genes. See Cornelissen and Melchers, “Strategies forControl of Fungal Diseases with Transgenic plants”, Plant Physiol.,101:709-712 (1993); and Bushnell et al., “Genetic Engineering of DiseaseResistance in Cereal”, Can. J. of Plant Path. 20(2):137-149 (1998).

2. Genes that Confer Resistance to an Herbicide, for Example:

A. An herbicide that inhibits the growing point or meristem, such as animidazolinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee et al.,EMBO J. 7:1241 (1988), and Miki et al., Theor. Appl. Genet. 80:449(1990), respectively.

B. Glyphosate (resistance conferred by mutant5-enolpyruvlshikimate-3-phosphate synthase (EPSPS) and aroA genes,respectively) and other phosphono compounds such as glufosinate(phosphinothricin acetyl transferase (PAT) and Streptomyceshygroscopicus PAT bar genes), and pyridinoxy or phenoxy proprionic acidsand cyclohexones (ACCase inhibitor-encoding genes). See, for example,U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotidesequence of a form of EPSPS which can confer glyphosate resistance. ADNA molecule encoding a mutant aroA gene can be obtained under ATCCaccession number 39256, and the nucleotide sequence of the mutant geneis disclosed in U.S. Pat. No. 4,769,061 to Comai. European patentapplication No. 0 333 033 to Kumada et al., and U.S. Pat. No. 4,975,374to Goodman et al., disclose nucleotide sequences of glutamine synthetasegenes which confer resistance to herbicides such as L-phosphinothricin.See also Russel, D. R., et al., Plant Cell Report, 12:3 165-169 (1993).The nucleotide sequence of a phosphinothricin-acetyl-transferase (PAT)gene is provided in European application No. 0 242 246 to Leemans et al.DeGreef et al., Bio/Technology 7:61 (1989) describe the production oftransgenic plants that express chimeric bar genes coding forphosphinothricin acetyl transferase activity. Exemplary of genesconferring resistance to phenoxy proprionic acids and cyclohexones, suchas sethoxydim and haloxyfop are the Acc1-S1, Acc1-S2, and Acc2-S3 genesdescribed by Marshall et al., Theor. Appl. Genet. 83:435 (1992).

C. An herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+ genes) and a benzonitrile (nitrilase gene). Przibila et al.,Plant Cell 3:169 (1991), describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker andDNA molecules containing these genes are available under ATCC AccessionNumbers. 53435, 67441 and 67442. Cloning and expression of DNA codingfor a glutathione S-transferase is described by Hayes et al., Biochem.J. 285:173 (1992).

D. Acetohydroxy acid synthase, which has been found to make plants thatexpress this enzyme resistant to multiple types of herbicides, has beenintroduced into a variety of plants. See Hattori et al., “AnAcetohydroxy acid synthase mutant reveals a single site involved inmultiple herbicide resistance”, Mol. Gen. Genet. 246:419-425, 1995.Other genes that confer tolerance to herbicides include a gene encodinga chimeric protein of rat cytochrome P4507A1 and yeast NADPH-cytochromeP450 oxidoreductase (Shiota et al., “Herbidcide-resistant Tobacco PlantsExpressing the Fused Enzyme between Rat Cytochrome P4501A1 (CYP1A1) andYeast NADPH-Cytochrome P450 Oxidoreductase”, Plant Physiol., 106:17,1994), genes for glutathione reductase and superoxide dismutase (Aono etal., “Paraquat tolerance of transgenic Nicotiana tabacum with enhancedactivities of glutathione reductase and superoxide dismutase”, PlantCell Physiol. 36:1687, 1995), and genes for various phosphotransferases(Datta et al., “Herbicide-resistant Indica rice plants from IRRIbreeding line IR72 after PEG-mediated transformation of protoplants”,Plant Mol. Biol. 20:619, 1992).

E. Protoporphyrinogen oxidase (protox) is necessary for the productionof chlorophyll, which is necessary for all plant survival. The protoxenzyme serves as the target for a variety of herbicidal compounds. Theseherbicides also inhibit growth of all the different species of plantspresent, causing their total destruction. The development of plantscontaining altered protox activity which are resistant to theseherbicides are described in U.S. Pat. Nos. 6,288,306; 6,282,837;5,767,373; and international publication WO 01/12825.

3. Genes that Confer or Contribute to a Value-Added Trait, Such As:

A. Delayed and attenuated symptoms to Bean Golden Mosaic Geminivirus(BGMV), for example by transforming a plant with antisens genes from theBrazilian BGMV. See Arago et al., Molecular Breeding. 1998, 4: 6,491-499.

B. Increased the bean content in Methionine by introducing a transgenecoding for a Methionine rich storage albumin (2S-albumin) from theBrazil nut as decribed in Arago et al., Genetics and Molecular Biology.1999, 22: 3, 445-449.

Methods for Garden Bean Transformation

Numerous methods for plant transformation have been developed includingbiological and physical plant transformation protocols. See, forexample, Miki et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glick,B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages67-88. In addition, expression vectors and in-vitro culture methods forplant cell or tissue transformation and regeneration of plants areavailable. See, for example, Gruber et al., “Vectors for PlantTransformation” in Methods in Plant Molecular Biology and Biotechnology,Glick, B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton,1993) pages 89-119.

A. Agrobacterium-mediated Transformation—One method for introducing anexpression vector into plants is based on the natural transformationsystem of Agrobacterium. See, for example, Horsch et al., Science227:1229 (1985); Diant et al., Molecular Breeding 3:1, 75-86 (1997). A.tumefaciens and A. rhizogenes are plant pathogenic soil bacteria whichgenetically transform plant cells. The Ti and Ri plasmids of A.tumefaciens and A. rhizogenes, respectively, carry genes responsible forgenetic transformation of the plant. See, for example, Kado, C. I.,Crit. Rev. Plant Sci. 10:1 (1991). Descriptions of Agrobacterium vectorsystems and methods for Agrobacterium-mediated gene transfer areprovided by Gruber et al., supra, Miki et al., supra and Moloney et al.,Plant Cell Reports 8:238 (1989). See also, U.S. Pat. No. 5,591,616issued Jan. 7, 1997.

B. Direct Gene Transfer—Despite the fact the host range forAgrobacterium-mediated transformation is broad, some cereal or vegetablecrop species and gymnosperms have generally been recalcitrant to thismode of gene transfer, even though some success has been achieved inrice and corn. Hiei et al., The Plant Journal 6:271-282 (1994) and U.S.Pat. No. 5,591,616 issued Jan. 7, 1997. Several methods of planttransformation, collectively referred to as direct gene transfer, havebeen developed as an alternative to Agrobacterium-mediatedtransformation. A generally applicable method of plant transformation ismicroprojectile-mediated transformation where DNA is carried on thesurface of microprojectiles measuring 1 to 4 microns. The expressionvector is introduced into plant tissues with a biolistic device thataccelerates the microprojectiles to speeds of 300 to 600 m/s which issufficient to penetrate plant cell walls and membranes. Russell, D. R.,et al. Pl. Cell. Rep. 12(3, January), 165-169 (1993), Aragao, F. J. L.,et al. Plant Mol. Biol. 20(2, October), 357-359 (1992), Aragao Theor.Appl. Genet 93: 142-150 (1996), Kim, J.; Minamikawa, T. Plant Science117: 131-138 (1996), Sanford et al., Part. Sci. Technol. 5:27 (1987);Sanford, J. C., Trends Biotech. 6:299 (1988); Klein et al., Bio/Tech.6:559-563 (1988); Sanford, J. C. Physiol Plant 7:206 (1990); Klein etal., Biotechnology 10:268 (1992).

Another method for physical delivery of DNA to plants is sonication oftarget cells. Zhang et al., Bio/Technology 9:996 (1991). Alternatively,liposome and spheroplast fusion have been used to introduce expressionvectors into plants. Deshayes et al., EMBO J., 4:2731 (1985); Christouet al., Proc Natl. Acad. Sci. USA 84:3962 (1987). Direct uptake of DNAinto protoplasts using CaCl₂ precipitation, polyvinyl alcohol orpoly-L-ornithine have also been reported. Hain et al., Mol. Gen. Genet.199:161 (1985) and Draper et al., Plant Cell Physiol. 23:451 (1982).Electroporation of protoplasts and whole cells and tissues have alsobeen described Saker, M and Kuhne, T. Biologia Plantarum 40(4): 507-514(1997/98); D'Halluin et al., Plant Cell 4:1495-1505 (1992) and Spenceret al., Plant Mol. Biol. 24:51-61 (1994)).

Following transformation of garden bean target tissues, expression ofthe above-described selectable marker genes allows for preferentialselection of transformed cells, tissues and/or plants, usingregeneration and selection methods well known in the art.

The foregoing methods for transformation would typically be used forproducing a transgenic variety. The transgenic variety could then becrossed with another (non-transformed or transformed) variety in orderto produce a new transgenic variety. Alternatively, a genetic trait thathas been engineered into a particular garden bean line using theforegoing transformation techniques could be moved into another lineusing traditional backcrossing techniques that are well known in theplant breeding arts. For example, a backcrossing approach could be usedto move an engineered trait from a public, non-elite variety into anelite variety, or from a variety containing a foreign gene in its genomeinto a variety or varieties that do not contain that gene. As usedherein, “crossing” can refer to a simple X by Y cross or the process ofbackcrossing depending on the context.

Backcrossing

When the term garden bean plant, cultivar or bean line are used in thecontext of the present invention, this also includes cultivars where oneor more desired traits has been introduced through backcrossing methods,whether such trait is a naturally occurring one or a transgenic one.Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the line. The term “backcrossing” asused herein refers to the repeated crossing of a hybrid progeny back tothe recurrent parent, i.e. backcrossing 1, 2, 3, 4, 5, 6, 7, 8, 9 ormore times to the recurrent parent. The parental bean plant whichcontributes the gene for the desired characteristic is termed thenonrecurrent or donor parent. This terminology refers to the fact thatthe nonrecurrent parent is used one time in the backcross protocol andtherefore does not recur. The parental bean plant to which the gene orgenes from the nonrecurrent parent are transferred is known as therecurrent parent as it is used for several rounds in the backcrossingprotocol.

In a typical backcross protocol, the original cultivar of interest(recurrent parent) is crossed to a second line (nonrecurrent parent)that carries the gene or genes of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a garden bean plantis obtained wherein essentially all of the desired morphological andphysiological characteristics of the recurrent parent are recovered inthe converted plant, generally determined at a 5% significance levelwhen grown in the same environmental condition, in addition to the geneor genes transferred from the nonrecurrent parent. It has to be notedthat some, one, two, three or more, self-pollination and growing ofpopulation might be included between two successive backcrosses. Indeed,an appropriate selection in the population produced by theself-pollination, i.e. selection for the desired trait and physiologicaland morphological characteristics of the recurrent parent might beequivalent to one, two or even three, additional backcrosses in acontinuous series without rigorous selection, saving time, money andeffort to the breeder. A non limiting example of such a protocol wouldbe the following: a) the first generation F₁ produced by the cross ofthe recurrent parent A by the donor parent B is backcrossed to parent A;b) selection is practiced for the plants having the desired trait ofparent B; c) selected plants are self-pollinated to produce a populationof plants where selection is practiced for the plants having the desiredtrait of parent B and the physiological and morphologicalcharacteristics of parent A; d) the selected plants are backcrossed one,two, three, four, five, six, seven, eight, nine or more times to parentA to produce selected backcross progeny plants comprising the desiredtrait of parent B and the physiological and morphologicalcharacteristics of parent A. Step c) may or may not be repeated andincluded between the backcrosses of step d.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalline. To accomplish this, a gene or genes of the recurrent cultivar ismodified or substituted with the desired gene from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphological,constitution of the original line. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross, one ofthe major purposes is to add some commercially desirable, agronomicalimportant trait to the plant. The exact backcrossing protocol willdepend on the characteristic or trait being altered to determine anappropriate testing protocol. Although backcrossing methods aresimplified when the characteristic being transferred is a single geneand dominant allele, multiple genes and recessive allele(s) may also betransferred and therefore, backcross breeding is by no means restrictedto character(s) governed by one or a few genes. In fact the number ofgenes might be less important that the identification of thecharacter(s) in the segregating population. In this instance it may thenbe necessary to introduce a test of the progeny to determine if thedesired characteristic(s) has been successfully transferred. Such testsencompass visual inspection, simple crossing but also follow up of thecharacteristic(s) through genetically associated markers and molecularassisted breeding tools. For example, selection of progeny containingthe transferred trait is done by direct selection, visual inspection fora trait associated with a dominant allele, while the selection ofprogeny for a trait that is transferred via a recessive allele requireselfing the progeny to determine which plant carry the recessiveallele(s).

Many single gene traits have been identified that are not regularlyselected for in the development of a new line but that can be improvedby backcrossing techniques. Single gene traits may or may not betransgenic, examples of these traits include but are not limited to,herbicide resistance (such as bar or pat genes), resistance forbacterial, fungal, or viral disease such as gene I used for BCMVresistance), insect resistance, enhanced nutritional quality (such as 2salbumine gene), industrial usage, agronomic qualities such as the“persistent green gene”, yield stability and yield enhancement. Thesegenes are generally inherited through the nucleus. Some other singlegene traits are described in U.S. Pat. Nos. 5,777,196; 5,948,957 and5,969,212, the disclosures of which are specifically hereby incorporatedby reference.

In 1981 the backcross method of breeding accounted for 17% of the totalbreeding effort for inbred corn line development in the United States,according to, Hallauer, A. R. et al. (1988) “Corn Breeding” Corn andCorn Improvement, No. 18, pp. 463-481.

The backcross breeding method provides a precise way of improvingvarieties that excel in a large number of attributes but are deficientin a few characteristics. (Page 150 of the Pr. R. W. Allard's 1960 book,published by John Wiley & Sons, Inc, Principles of Plant Breeding). Themethod makes use of a series of backcrosses to the variety to beimproved during which the character or the characters in whichimprovement is sought is maintained by selection. At the end of thebackcrossing the gene or genes being transferred unlike all other genes,will be heterozygous. Selfing after the last backcross produceshomozygosity for this gene pair(s) and, coupled with selection, willresult in a variety with exactly the adaptation, yielding ability andquality characteristics of the recurrent parent but superior to thatparent in the particular characteristic(s) for which the improvementprogram was undertaken. Therefore, this method provides the plantbreeder with a high degree of genetic control of his work.

The backcross method is scientifically exact because the morphologicaland agricultural features of the improved variety could be described inadvance and because the same variety could, if it were desired, be breda second time by retracing the same steps (Briggs, “Breeding wheatsresistant to bunt by the backcross method”, 1930 Jour. Amer. Soc.Agron., 22: 289-244).

Backcrossing is a powerful mechanism for achieving homozygosity and anypopulation obtained by backcrossing must rapidly converge on thegenotype of the recurrent parent. When backcrossing is made the basis ofa plant breeding program, the genotype of the recurrent parent will bemodified only with regards to genes being transferred, which aremaintained in the population by selection.

Successful backcrosses are for example, the transfer of stem rustresistance from “Hope” wheat to “Bart” wheat and even pursuing thebackcrosses with the transfer of bunt resistance to create “Bart 38”,having both resistances. Also highlighted by Allard is the successfultransfer of mildew, leaf spot and wilt resistances in “CaliforniaCommon” alfalfa to create “Caliverde”. This new “Caliverde” varietyproduced through the backcross process is indistinguishable from“California Common” except for its resistance to the three nameddiseases.

One of the advantages of the backcross method is that the breedingprogram can be carried out in almost every environment that will allowthe development of the character being transferred.

The backcross technique is not only desirable when breeding for diseaseresistance but also for the adjustment of morphological characters,color characteristics and simply inherited quantitative characters suchas earliness, plant height and seed size and shape. In this regard, amedium grain type variety, “Calady”, has been produced by Jones andDavis. As dealing with quantitative characteristics, they selected thedonor parent with the view of sacrificing some of the intensity of thecharacter for which it was chosen, i.e. grain size. “Lady Wright”, along grain variety was used as the donor parent and “Coloro”, a shortgrain one as the recurrent parent. After four backcrosses, the mediumgrain type variety “Calady” was produced.

TABLES

In Tables 2 and 3 that follow, the traits and characteristics of gardenbean cultivar H25101 are compared to the ‘Hystyle’ variety of gardenbean. The data was collected in 2006 and 2007 in several USA locations.

The first column shows the variety.

The second column shows the location of testing. “Coloma” indicatesColoma, Wisconsin; “SunPrairie” indicates Sun Prairie, Wis.; “Salem”indicates Salem, Oreg.; and “Arlington” indicates Arlington, Wis.

The third column shows the plant height in inches.

The forth column shows the plant width in inches.

The fifth column indicates the pod length in centimeters.

The sixth column shows the relative pod color with 1=light and 9=dark.

The seventh column shows the relative number of days to edible pods.

TABLE 2 Characteristic Comparisons for 2006 Relative Pod Color days toPlant Height Plant Width Pod length 1 = light edible VARIETY LOCATION(inches) (inches) (cm) 9 = dark pods HMX 5101 06SunPrairie2 21 22 15 660 06Coloma2 19 23 15 5 58 06Arlington1 19 22 15 5 63 06Coloma3 17 25 145 61 06Coloma1 20 22 15 5 60 06SunPrairie1 18 20 13 6 06Salem2 20 20 135 68 06SunPrairie3 17 22 15.5 6 06Arlington2 20 24 15 6 54 average 19.022.2 14.5 5.4 60.6 HYSTYLE 06SunPrairie3 17.0 21.0 16.0 4.0 06Arlington117.0 21.0 14.0 4.0 06SunPrairie1 16.0 18.0 13.5 3.0 06Arlington2 18.024.0 14.0 4.0 59.0 06Coloma3 19.0 28.0 14.0 3.0 61.0 06Salem2 20.0 21.013.0 4.0 66.0 06Coloma2 20.0 23.0 14.0 4.0 58.0 06SunPrairie2 20.0 21.014.5 3.0 60.0 average 18.4 22.1 14.1 3.6 60.8

TABLE 3 Characteristic Comparisons for 2007 Relative Pod Color days toPlant Height Plant Width Pod length 1 = light edible VARIETY LOCATION(inches) (inches) (cm) 9 = dark pods HMX 5101 07Coloma2 22 26 15 5 6007Coloma3 19 23 16 5 63 07Coloma1 20 23 16 6 64 07Arlington1 20 24 13.56 57 07SunPrairie1 20 22 16 5 54 07SunPrairie2 16 7 average 20.2 23.615.4 5.7 59.6 Hystyle 07Coloma3 19 22 14.5 4 61 07SunPrairie1 17 23 15 455 07Coloma2 23 25 14 4 59 07SunPrairie2 14 4 07Coloma1 20 24 15 3 6307Arlington1 18 23 15 4 57 average 19.4 23.4 14.6 3.8 59.0

DEPOSIT INFORMATION

A deposit of the Harris Moran Seed Company proprietary garden bean namedH25101 disclosed above and recited in the appended claims has been madewith the National Collections of Industrial, Food and Marine Bacteria(NCIMB), NCIMB Ltd., Ferguson Building, Craibstone Estate, Bucksburn,Aberdeen AB21 9YA, Scotland, UK, The date of deposit was May 1, 2009,The deposit of 2,500 seeds was taken from the same deposit maintained byHarris Moran Seed Company since prior to the filing date of thisapplication. All restrictions upon the deposit will be removed upongranting of a patent, and the deposit is intended to meet all of therequirements of 37 C.F.R. §1.801-1.809. The NCIMB accession number isNCIMB No. 41620. The deposit will be maintained in the depository for aperiod of 30 years, or 5 years after the last request, or for theeffective life of the patent. whichever is longer. and will be replacedas necessary during that period.

While a number of exemplary aspects and embodiments have been discussedabove, those of skill in the art will recognize certain modifications,permutations, additions and sub-combinations thereof. It is thereforeintended that the following appended claims and claims hereafterintroduced are interpreted to include all such modifications,permutations, additions and sub-combinations as are within their truespirit and scope.

1. A seed of bean cultivar designated H25101, wherein a representativesample of seed of said cultivar has been deposited under NCIMB No.41620.
 2. A bean plant, or a part thereof, produced by growing the seedof claim
 1. 3. A bean plant, or a part thereof, having all thephysiological and morphological characteristics of bean cultivar H25101listed in Table
 1. 4. A bean plant, or a part thereof, having thephysiological and morphological characteristics of bean cultivar H25101,wherein a representative sample of seed of said cultivar has beendeposited under NCIMB No.
 41620. 5. A tissue culture of regenerablecells produced from the plant of claim 2 wherein said cells of thetissue culture are produced from a plant part selected from the groupconsisting of embryos, meristematic cells, leaves, pollen, root, roottips, stems, anther, pistils, pods, flowers or seeds.
 6. A bean plantregenerated from the tissue culture of claim 5, said plant having themorphological and physiological characteristics of bean cultivar H25101,wherein a representative sample of seed has been deposited under NCIMBNo.
 41620. 7. A method for producing a bean seed comprising crossing afirst parent bean plant with a second parent bean plant and harvestingthe resultant hybrid bean seed, wherein said first parent bean plant orsecond parent bean plant is the bean plant of claim
 2. 8. A hybrid beanseed produced by the method of claim
 7. 9. A method for producing anherbicide resistant bean plant comprising transforming the bean plant ofclaim 2 with a transgene that confers herbicide resistance to anherbicide selected from the group consisting of imidazolinone,sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, andbenzonitrile.
 10. An herbicide resistant bean plant, or a part thereof,produced by the method of claim
 9. 11. A method for producing an insectresistant bean plant comprising transforming the bean plant of claim 2with a transgene that confers insect resistance.
 12. An insect resistantbean plant, or a part thereof, produced by the method of claim
 11. 13. Amethod for producing a disease resistant bean plant comprisingtransforming the bean plant of claim 2 with a transgene that confersdisease resistance.
 14. A disease resistant bean plant, or a partthereof, produced by the method of claim
 13. 15. A method of introducinga desired trait into bean cultivar H25101 comprising: (a) crossing abean cultivar H25101 plant grown from bean cultivar H25101 seed, whereina representative sample of seed has been deposited under NCIMB No.41620, with another bean plant that comprises a desired trait to produceF₁ progeny plants, wherein the desired trait is selected from the groupconsisting of insect resistance, disease resistance, water stresstolerance, heat tolerance, improved shelf life and improved nutritionalquality; (b) selecting one or more progeny plants that have the desiredtrait to produce selected progeny plants; (c) crossing the selectedprogeny plants with the bean cultivar H25101 plants to produce backcrossprogeny plants; (d) selecting for backcross progeny plants that have thedesired trait and physiological and morphological characteristics ofbean cultivar H25101 listed in Table 1 to produce selected backcrossprogeny plants; and (e) repeating steps (c) and (d) three or more timesin succession to produce selected fourth or higher backcross progenyplants that comprise the desired trait and the physiological andmorphological characteristics of bean cultivar H25101 listed in Table 1.16. A bean plant produced by the method of claim 15, wherein the planthas the desired trait and the all physiological and morphologicalcharacteristics of bean cultivar H25101 listed in Table
 1. 17. A methodfor producing bean cultivar H25101 seed, wherein a representative sampleof seed has been deposited under NCIMB No. 41620, comprising crossing afirst parent bean cultivar with a second parent bean cultivar andharvesting the resultant bean seed, wherein both said first and secondbean cultivars are the bean cultivar of claim
 4. 18. The bean plant ofclaim 16, wherein the desired trait is herbicide resistance and theresistance is conferred to an herbicide selected from the groupconsisting of imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine and benzonitrile.
 19. The bean plant ofclaim 16, wherein the desired trait is insect resistance and the insectresistance is conferred by a transgene encoding a Bacillus thuringiensisendotoxin.
 20. The bean plant of claim 16, wherein the desired trait iselected from the group consisting of insect resistance, diseaseresistance, water stress tolerance, heat tolerance, improved shelf lifeand improved nutritional quality.